The applicability of genetically modified microorganisms in bioremediation of contaminated environments
WASILKOWSKI D., SWĘDZIOŁ Ż., MROZIK A.
Please cite as: CHEMIK 2012, 66, 8, 817-826
Genetic engineering is a modern technology, which allows to design microorganisms capable of degrading specific contaminants. The construction of GMMs for bioremediation purposes is possible because many degradative pathways, enzyme and their respective genes are known and biochemical reactions are well understood. For selection and identification of GMMs in the environment many molecular techniques were developed. They include FISH, in situ PCR, DGGE, TGGE, T-RFLP, ARDRA and marker genes (lux, gfp, lacZ, xylE). In order to reduce potential risk of the use of GMMs in the environment some genetic barriers were created. They limit survival of the recombinants and gene transfer into autochthonous microorganisms. In this review the construction and practical applications of GMMs in bioremediation studies are discussed.
Keywords: GMMs, contaminants, degradation, bioremediation